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Chapter 2 Installing the Voyager™ Biospectrometry™ Workstations
2-42 PerSeptive Biosystems
2
What you need
To align the sample plate you need the following materials:
Sample plate with
α
-cyano-4-hydroxycinnamic acid
(CHCA) matrix spotted in four corner sample positions, as
described in Table 2-1. For more information, see
Section 3.5.4, Adjusting the Laser Position for a Custom
.PLT File.
NOTE: If your monitor screen is marked with the
position at which the laser strikes the sample plate and
you are aligning plates with wells or etched sample
positions, you do not need to spot the sample plate
with matrix before aligning. You can align the
boundaries of the sample positions with the laser
position mark on your monitor.
Transparency (mylar overhead or acetate)
Marking pen
NOTE: View the video monitor from a consistent angle
when determining laser positions.
Corner positions
in .PLT files
Different sample position numbers are associated with
different .PLT files. To determine which numbers correspond
to the four corners of each .PLT file, see Table 2-1.
Table 2-1 Four-Corner Positions on Sample Plates
.PLT file
Four Corner Position
Numbers
64 well disposable plate.PLT 82, 12, 19, 89
100 well plate.PLT 91, 11, 19, 99
384 well plate.PLT P1, C1, C21, P21
400 well plate.PLT T1, A1, A20, T20
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