Voyager DP-263-S Podręcznik Użytkownika Pobierz pdf (Strona 8)

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Table of Contents

viii PerSeptive Biosystems

Chapter 6 Acquiring Mass Spectra

6.1 Before You Begin.................................................................... 6-2

6.1.1 Overview of Acquisition Options ............................................. 6-2

6.1.2 Guidelines for Acquiring ......................................................... 6-4

6.1.3 Calibrating the Mass Scale .................................................... 6-7

6.2 Acquiring in Manual Mode from the Instrument Control Panel

........... 6-11

6.2.1 Manually Acquiring, Evaluating, and Saving Spectra .............6-11

6.2.2 Manually Accumulating Spectra

from Multiple Acquisitions .....................................................6-19

6.3 Obtaining Good Spectra in Delayed Extraction Mode

...................... 6-20

6.3.1 What is a Good Spectrum? ...................................................6-20

6.3.2 Laser Intensity ......................................................................6-23

6.3.3 Parameters Affecting Resolution and

Signal-to-Noise Ratio ............................................................6-24

6.4 Making Accurate Mass Measurements

........................................ 6-25

6.5 Evaluating Data in the Instrument Control Panel

............................ 6-27

6.5.1 Detecting, Integrating, and Labeling Peaks ...........................6-27

6.5.2 Calculating Mass Resolution .................................................6-29

6.5.3 Calculating Signal-to-RMS Noise Ratio .................................6-32

6.6 Acquiring in Automatic Mode from the Instrument Control Panel

........ 6-34

6.6.1 Before Acquiring in Automatic Control Mode .........................6-34

6.6.2 Setting Instrument Settings for Automatic Control Mode .......6-35

6.6.3 Automatically Acquiring, Evaluating, and

Saving Spectra .....................................................................6-43

6.6.4 Search Pattern Files .............................................................6-44

6.6.5 Process that Occurs During Acquisition

in Automatic Mode ................................................................6-49

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