Voyager DP-263-S Podręcznik Użytkownika Pobierz pdf (Strona 184)

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Chapter 3 Preparing Samples

3-54 PerSeptive Biosystems

X and Y Coordinates of the position center in reference to the lower left corner

of the sample plate. To determine the coordinates to enter in the .PLT

file, display the Sample View (see Figure 4-9 on page 4-31) and

record the Absolute X, Absolute Y (logical coordinates) pairs for each

position specified. See “Logical Coordinates on Plate View” on

page 3-61 for information on displaying logical coordinates.

NOTE: On a standard 100-well plate, position 91 is located at

X=1587.5, Y=1587.5, and corresponding to the lower left of the

sample plate.

For optimum mass accuracy, do not specify coordinates on the outer

edges of a plate.

The following coordinates correspond to the corner positions that

define the area of the sample plate that provides optimum mass

accuracy (illustrated in Figure 3-11 on page 3-56):

• Absolute X=6667.5, Absolute Y=42227.5—Top left (corresponds

to position 1)

• Absolute X=42227.5, Absolute Y=42227.5—Top right

• Absolute X=6667.5, Absolute Y=6667.5—Bottom left

• Absolute X=42227.5, Absolute Y=6667.5—Bottom right

NOTE: Sample plate alignment for custom plate types (.PLT files that

you create) requires sample spots on any four corner positions. You

can use any four corner positions, but for maximum mass accuracy,

do not use positions outside the area defined by the coordinates

above.

Position

name

Displayed in the Active Position list in the Sample View. Optional.

NOTE: If you do not specify a position name in the file, the position

number is displayed. The first sample in the .PLT file is considered

sample 1. The Nth sample in the .PLT file is considered sample N.

Table 3-4 .PLT File Parameters (Continued)

Parameter Description

1 2 ... 179 180 181 182 183 184 185 186 187 188 189 ... 611 612

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