Voyager DP-263-S Podręcznik Użytkownika Pobierz pdf (Strona 150)

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101

Chapter 3 Preparing Samples

3-20 PerSeptive Biosystems

3.1.5.1 Washing

When to use

Use this technique when you know the solubility of the

contaminant. You can wash a dried sample directly on the

sample plate.

What you need

Select a solvent in which the contaminant is more soluble than

the matrix and the sample. For example, use:

• Cold water (to prevent sample dissolving) with 0.1% TFA

for a polar contaminant, such as a salt or buffer

• 5% isopropanol in water for a non-ionic detergent such as

octyl glucoside

Procedure

To wash:

1. Apply sample and matrix to the sample plate and allow

to dry.

2. Add 1 to 3 µl of solvent to the dry sample/matrix.

3. Wait 10 seconds, then remove the liquid.

NOTE: Try not to touch the sample plate with the pipet

tip. If you do touch the spot, do not analyze the area

you touched.

4. Repeat step 2 and step 3 one more time.

5. Allow the sample plate to dry before analyzing.

If one wash does not improve results, you can wash

again.

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