Voyager DP-263-S Podręcznik Użytkownika Pobierz pdf (Strona 40)

100

101

Chapter 1 Introducing the Voyager™ Biospectrometry™ Workstations

1-10 PerSeptive Biosystems

Drift time is proportional to the square root of the mass as

defined by the following equation:

Approximate ion mass is determined using the equation

above. A calibration procedure using a reference standard of

known mass can be used to establish a more accurate

relationship between flight time and the mass-to-charge ratio

of the ion.

Advantages of

MALDI-TOF

Advantages of MALDI-TOF technology include:

• Can analyze a theoretically unlimited mass range, greater

than 300,000 daltons (Da). Mass range is limited by

ionization ability, not analyzer physics.

• Complete mass spectrum can be obtained from a single

ionization event. This is also referred to as multiplexing,

or parallel (versus serial) detection.

• Is compatible with buffers normally used in biological

assays, reducing the need for sample cleanup.

• Can analyze mixtures and different classes of

biopolymers, including peptides, oligonucleotides,

glycoconjugates, and synthetic polymers.

• Provides very high sensitivity and requires only

femtomoles of sample.

t = s

(2KE)z

()

where:

t drift time=

s = drift distance

m=mass

KE =

kinetic energy

z = number of charges on ion

1 2 ... 35 36 37 38 39 40 41 42 43 44 45 ... 611 612

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